| Giornata Nº 21 |
| Data | Incontro |
| 03/04/2026 | Vysocina Jihlava | Prostejov |
| 03/04/2026 | FK Usti Nad Labem | FC Silon Taborsko |
| 03/04/2026 | Graffin | Opava |
| 04/04/2026 | Brno | MFk Chrudim |
| 04/04/2026 | SK Hanacka Slavia Kromeriz | Artis |
| 05/04/2026 | Slavia Prague B | Zizkov |
| 05/04/2026 | Sparta Praga B | Budejovice |
| 05/04/2026 | FC Banik Ostrava B | Pribram |
| Giornata Nº 22 |
| Data | Incontro |
| 10/04/2026 | Prostejov | FC Banik Ostrava B |
| 10/04/2026 | FC Silon Taborsko | Sparta Praga B |
| 10/04/2026 | Budejovice | FK Usti Nad Labem |
| 10/04/2026 | Pribram | SK Hanacka Slavia Kromeriz |
| 11/04/2026 | MFk Chrudim | Slavia Prague B |
| 11/04/2026 | Opava | Brno |
| 11/04/2026 | Artis | Graffin |
| 12/04/2026 | Zizkov | Vysocina Jihlava |
